• Extracting Apple Juice with Pectinase
  
• Simple Enzyme Experiments
  
• A Pectinase Class Activity
  
• Learn how the enzyme pectinase breaks down the pectin found in plant cell walls
  
• Apple juice with pectinase (video)
  
• Pectinase assay
  

Pectinase is a general term for enzymes that break down pectin, a polysaccharide substrate that is found in the cell walls of plants. One of the most studied and widely used commercial pectinases is polygalacturonase. It is useful because pectin is the jelly-like matrix which helps cement plant cells together and in which other cell wall components, such as cellulose fibrils, are embedded. Therefore pectinase enzymes are commonly used in processes involving the degradation of plant materials, such as speeding up the extraction of fruit juice from fruit, including apples and sapota. Pectinases have also been used in wine production since the 1960s. They can be extracted from fungi such as Aspergillus niger. The fungus produces these enzymes to break down the middle lamella in plants so that it can extract nutrients from the plant tissues and insert fungal hyphae. If pectinase is boiled it is denatured (distorted) making it harder to connect with the pectin at the active site, and produce as much juice.

Pectinases are also used for retting. Addition of chelating agents or pretreatment of the plant material with acid enhance the effect of the enzyme.

Optimum environment

As they are enzymes, pectinases have an optimum temperature and pH at which they are most active. For example, a commercial pectinase might typically be activated at 45 to 55 °C and work well at a pH of 4.0 to 5. Pectinases are useful in fruit juice industries... Enzymes are biological catalysyts that speed up chemical reactions by lowering their activation energy.

External links

• Pectinase Database website - Gives complete information of pectinase enzymes and their structures, classifications, specific activities, literature, drug design, list of industries, etc. ^[1]

References